Abstract
Peak splitting due to the residual dipolar coupling (RDC) represents a potentially applicable spectral parameter for diagnostic purposes. Several of the skeletal muscle metabolites were previously reported to display the RDC splitting inin vivoMR spectra. We constructed anin vitromodel consisting of mechanically stretched gelatin cylinder soaked with the muscle metabolite carnosine. We describe the preparation procedure of an upscaled 50 mL stretched gelatin sample with carnosine that can be used as a phantom for setting-up and testing of spectroscopic measurements of RDC in a MR scanner. We also report on analysis of the RDC splittings in1H and13C high resolution MR spectra of carnosine.
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