Abstract
Abstract The choosing of micromechanical environment is very important for the growth of bone-related cells. In this paper, bovine cancellous bone slices with 3D porous structures were used for 3D culturing of MC3T3-E1 cells (Mouse embryo osteoblast precursor cells) through a four-point-bending device due to their good biocompatibility and strength. Effects of micromechanical environment on the growth of MC3TC-E1 cells were investigated by immunofluorescent staining and alkaline phosphatase analysis, and the most positive microporous structures were found. In addition, a model of cell density vs stress was established through a specific normalization method and finite element simulation. The results showed that the micromechanical environment of the bone slices promoted cell proliferation, and the detail influence of stress on cell proliferation could be described by the mathematical model, which could provide a theoretical basis for the design of micromechanical environment in the bone tissue engineering scaffolds to stimulate cell proliferation.
Highlights
The choosing of micromechanical environment is very important for the growth of bone-related cells
Total alkaline phosphatase (ALP) content which represented ALP content secreted by cells from the beginning to the 2nd, 4th, 6th, and 8th days was acquired through accumulating the average ALP content on different days
The results showed that the average values of cell density in the stressed group were higher than those in the control group, indicating that stress can promote the proliferation of cells
Summary
Abstract: The choosing of micromechanical environment is very important for the growth of bone-related cells. Bovine cancellous bone slices with 3D porous structures were used for 3D culturing of MC3T3-E1 cells (Mouse embryo osteoblast precursor cells) through a four-point-bending device due to their good biocompatibility and strength. Effects of micromechanical environment on the growth of MC3TC-E1 cells were investigated by immunofluorescent staining and alkaline phosphatase analysis, and the most positive microporous structures were found. A model of cell density vs stress was established through a specific normalization method and finite element simulation. The results showed that the micromechanical environment of the bone slices promoted cell proliferation, and the detail influence of stress on cell proliferation could be described by the mathematical model, which could provide a theoretical basis for the design of micromechanical environment in the bone tissue engineering scaffolds to stimulate cell proliferation
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