Streptozotosin ile Diyabet Oluşturulmuş ve Metformin ile İnsülin Uygulanan Ratlarda Oleuropein'in Çeşitli Dokularda Nitrik Oksit, Glutatyon, Malondialdehit Düzeyleri ve Glutatyon Peroksidaz Aktivitesi Üzerine Etkileri

Abstract

Objective: The aim of the present study was to investigate the effects of oleuropein on nitric oxide (NO) and oxidant-antioxidant levels in tissues of streptozotocin-induced diabetic rats treated with metformin and insulin. Material and Methods: A total of 40 male Sprague-Dawley rats were used in this study. Animals were divided into 5 groups (n=8) including 3 experiment and 2 control groups. Saline solution was injected intraperitoneally (IP) to the rats in control group. 50 mg/kg STZ was given IP to the Diabetic Control, 50 mg/kg streptozotocin (STZ)+100 mg/kg Metformin+4 IU/kg insulin was given IP to Group 1, 50 mg/kg STZ+100 mg/kg Metformin+4 IU/kg insulin was given IP and 30 mg/kg oleuropein was given orally to Group 2 and 50 mg/kg STZ IP and 30 mg/kg oleuropein was given orally to Group 3. Results: Metformin and insulin with oleuropein have a synergistic effect on regulatory activity of the GSH, MDA, NO levels and GSHPx activities of the kidney, brain and heart tissues. Using metformin and insulin with oleuropein increased the GSH levels of the kidney (p<0.001), brain (p<0.001) and heart tissues (p<0.05); also increased the GSHPx activity of the brain (p<0.01) and the heart tissues (p<0.001) and reduced MDA and NO levels of the kidney, brain and heart tissues (p<0.001). There were no statistically significant differences in GSHPx activity of kidney tissues of experimental groups. Conclusion: Using oleuropein, on streptozotocin-diabetics, in combination with metformin and insulin seems to have synergistic effect on the antioxidants in kidney, brain and heart tissues and effective in regulating the NO level on tissues.