Streptomyces spp. Biofilmed Solid Inoculant Improves Microbial Survival and Plant-Growth Efficiency of Triticum aestivum

Abstract

Streptomyces species have been successfully used in diverse biotechnological processes; however, little is known about actinobacterial biofilm formation and its use as a biofilmed inoculant. The present study assessed and compared the ability of some plant growth-promoting actinobacterial strains to form biofilms on a carrier to improve microbial survival and colonize the rhizosphere and roots of Triticum aestivum, resulting in positive interactions and benefits to the plant. Forty-one actinobacterial isolates from Persea americana organic rhizosphere farms were tested on plant growth and biofilm-forming capacities, showing their potential use as bio-fertilizers in agriculture. Three Streptomyces strains were selected and tested for biofilm formation and plant growth-promoting (PGP) features. Biofilms were induced on the perlite carrier and used to inoculate seven treatments with T. aestivum in pot essays, resulting in a >200% increase in root weight and a >400% increase in total biomass. Endophytic colonization was achieved in all the treatments. Microbial survival ranged between 108 and 109 CFU/g after 12 weeks of treatment, indicating actinobacterial permanence on the carrier. Successful consortia formation was observed for mixed-strain treatments, suggesting long-term plant recolonization.