Streptomyces sasae sp. nov., isolated from bamboo (Sasa borealis) rhizosphere soil.

Abstract

A novel strain of Gram-staining-positive actinobacterium, designated strain JR-39T, was isolated from the rhizosphere soil of bamboo (Sasa borealis) sampled in Damyang, Korea, and its taxonomic position was investigated by a polyphasic approach. The isolate formed flexuous chains of spores that were cylindrical and smooth-surfaced. Strain JR-39T grew at 4–37 °C (optimum 28 °C). The pH range for growth was pH 5–10 (optimum pH 6–8) and the NaCl range for growth was 0–5 % (w/v) with optimum growth at 1 % NaCl. The cell-wall peptidoglycan contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates mainly contained glucose, mannose, ribose and rhamnose. Predominant menaquinones were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0. The G+C content of the DNA was 72.3 ± 0.34 mol%. Phylogenetic analyses based on 16S rRNA gene sequence analysis indicated that strain JR-39T belonged to the genus Streptomyces, showing the highest sequence similarity to Streptomyces panaciradicis 1MR-8T (99.4 %), Streptomyces capoamus JCM 4734T (98.8 %), Streptomyces galbus DSM 40089T (98.7 %), Streptomyces longwoodensis LMG 20096T (98.7 %), Streptomyces bungoensis NBRC 15711T (98.7 %) and Streptomyces rhizophilus JR-41T (98.7 %). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain JR-39T could be differentiated from its closest phylogenetic relatives. On the basis of the phenotypic and genotypic characteristics, strain JR-39T represents a novel species for which the name Streptomyces sasae sp. nov. is proposed. The type strain is JR-39T ( = KACC 17182T = NBRC 109809T).