Streptomyces pactum combined with manure compost alters soil fertility and enzymatic activities, enhancing phytoextraction of potentially toxic metals (PTMs) in a smelter-contaminated soil

Abstract

The effect of manure compost alone and combined with Streptomyces pactum (Act12) applied in the smelter-contaminated soil was investigated. The soil fertility, enzymatic activities, potentially toxic metals (PTMs) solubility, and phytoremediation efficiency of potherb mustard (Brassica juncea, Coss.) were assessed. Results showed that the application of compost reduced the soil pH, while significantly increased the soil electrical conductivity (EC) (7.0 folds), available phosphorus (AP) (10.8 folds), available potassium (AK) (2.81 folds), dissolved organic carbon (DOC) (5.22 folds), organic matter (OM) (4.93 folds), together with soil enzymatic activities viz. urease (UR) (4.39 folds), dehydrogenase (DEH) (45.0 folds) and alkaline phosphatase (ALP) (123.9 folds) in comparison with control. The inoculation of Act12 increased AP, AK, DOC, OM and UR values, but reduced EC, DEH and ALP values compared to corresponding lone compost amendment. Additionally, Act12 solubilized PTMs (Cd and Zn) in the soil, and accordingly enhanced the PTMs uptake in the plant. The phytoextraction indices viz. biological concentration factor (BCF), translocation factor (TF) and metal extraction amount (MEA) indicated that compost and Act12 had a synergistic role in enhancing the phytoremediation efficiency, among which MEA values of Cd and Zn maximally increased by 9.64 and 11.4 folds, respectively, compared to control. Redundancy analysis (RDA) indicated that phytoextraction indices correlated well with soil parameters. Our results suggested that manure compost associated with Act12 is a potential strengthening strategy in phytoremediation of PTMs contaminated soil.