Abstract
(1) Background: Streptomyces sp. TP-A0598 derived from seawater produces lydicamycin and its congeners. We aimed to investigate its taxonomic status; (2) Methods: A polyphasic approach and whole genome analysis are employed; (3) Results: Strain TP-A0598 contained ll-diaminopimelic acid, glutamic acid, glycine, and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H6) and MK-9(H8), and the major fatty acids were C16:0, iso-C15:0, iso-C16:0, and anteiso-C15:0. Streptomyces sp. TP-A0598 showed a 16S rDNA sequence similarity value of 99.93% (1 nucleottide difference) to Streptomyces angustmyceticus NRRL B-2347T. The digital DNA–DNA hybridisation value between Streptomyces sp. TP-A0598 and its closely related type strains was 25%–46%. Differences in phenotypic characteristics between Streptomyces sp. TP-A0598 and its phylogenetically closest relative, S. angustmyceticus NBRC 3934T, suggested strain TP-A0598 to be a novel species. Streptomyces sp. TP-A0598 and S. angustmyceticus NBRC 3934T harboured nine and 13 biosynthetic gene clusters for polyketides and nonribosomal peptides, respectively, among which only five clusters were shared between them, whereas the others are specific for each strain; and (4) Conclusions: For strain TP-A0598, the name Streptomyces lydicamycinicus sp. nov. is proposed; the type strain is TP-A0598T (=NBRC 110027T).
Highlights
The genus Streptomyces is the largest taxon within the phylum Actinobacteria, and the members are an attractive source of bioactive secondary metabolites
Genome analyses of Streptomyces strains revealed that each strain has a large and linear chromosome encoding more than 20 secondary metabolite biosynthetic gene clusters, even if it is known to produce only few secondary metabolites
Because polyketides and nonribosomal peptides show various bioactivities, genome mining focused on polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters often leads to the discovery of new biologically active compounds
Summary
The genus Streptomyces is the largest taxon within the phylum Actinobacteria, and the members are an attractive source of bioactive secondary metabolites. Genome analyses of Streptomyces strains revealed that each strain has a large and linear chromosome encoding more than 20 secondary metabolite biosynthetic gene clusters (smBGCs), even if it is known to produce only few secondary metabolites. This means that hitherto reported compounds are nothing more than only a part of the secondary metabolites that they can produce. Because polyketides and nonribosomal peptides show various bioactivities, genome mining focused on PKS and NRPS gene clusters often leads to the discovery of new biologically active compounds. We discuss the similarity and difference of these smBGCs among taxonomically close strains
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
