Streptomyces klenkii sp. nov., isolated from deep marine sediment.

Abstract

A novel actinomycete strain, designated S2704(T), was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey and characterized using a polyphasic approach. Comparison of 16S rRNA gene sequences showed that strain S2704(T) is a member of the genus Streptomyces, exhibiting highest similarity with Streptomyces specialis GW 41-1564(T) (97.1%). Strain S2704(T) could be distinguished from all other Streptomyces species with validly published names by sequence similarity values less than 97.0%. The isolate was found to contain LL-diaminopimelic acid as the diagnostic diamino acid. Whole cell hydrolysates were found to contain glucose, mannose and ribose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, glycophospholipid, a phospholipid and two unknown glycolipids. The predominant menaquinones were identified as MK-9(H8) and MK-10(H6). The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and anteiso-C15:0. The G+C content of the DNA was determined to be 72.2mol%. On the basis of its phenotypic and molecular properties, strain S2704(T) is considered to be a novel species of the genus Streptomyces, for which the name Streptomyces klenkii sp. nov. is proposed. The type strain is S2704(T) (=DSM 42104(T)=KCTC 29202(T)).