Abstract
In order to explore the biocontrol potential of Streptomyces felleus YJ1 to sclerotinia stem rot of oilseed rape, we evaluated the effects of YJ1 as antagonistic strain on Sclerotinia sclerotiorum, including germination and formation of sclerotia, ascospore germination, mycelial growth and YJ1 colonization ability. We found the fermentation filtrate of YJ1 could inhibit sclerotia and ascospore germination and mycelial growth. In dual culture the inhibition zone diameter of YJ1 against S. sclerotiorum was 11.0 mm, and the inhibition rate reached to 80.26%. The ascospores germination was also significantly inhibited by YJ1 fermentation filtrate. In addition, YJ1 could colonize stably in rhizosphere and roots of rape. Otherwise, in the greenhouse we found the lesion would become smaller and slighter if the inoculated leaves were pretreated with YJ1 fermentation liquid. Therefore, our results strongly suggested that YJ1 was a promising biocontrol agent for control of oilseed rape sclerotinia stem rot.
Highlights
Sclerotinia stem rot of oilseed rape caused by the fungus Sclerotinia sclerotiorum (Lib.) de Bary is a widespread soil-borne plant disease (Yan et al, 2005)
In order to explore the biocontrol potential of Streptomyces felleus YJ1 to sclerotinia stem rot of oilseed rape, we evaluated the effects of YJ1 as antagonistic strain on Sclerotinia sclerotiorum, including germination and formation of sclerotia, ascospore germination, mycelial growth and YJ1 colonization ability
This indicated that YJ1 had obvious antagonism against S. sclerotiorum mycelial growth (Figure 1)
Summary
Sclerotinia stem rot of oilseed rape caused by the fungus Sclerotinia sclerotiorum (Lib.) de Bary is a widespread soil-borne plant disease (Yan et al, 2005). The fungus is capable of infecting a wide variety of plant species including soybean, bean, pea, lettuce, tomato, sunflower, rape and many other economically important crops (Hegedus & Rimmer, 2005; Fróes et al, 2012). Oilseed rape (Brassica napus L.) is the major oil crop in China. S. sclerotiorum has an increasing threat to oilseed rape cultivation and become a potentially damaging disease. Sclerotinia stem rot can reduce the yield of oilseed rape from 15% to 60% (Subbarao, 1998). Due to its wide host-range, effective reproduction and sclerotia which can survive in soil for up to four years without hosts or favorable condition, it is very difficult to control S. sclerotiorum (Bae & Knudsen, 2007)
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