Streptomyces dengpaensis sp. nov., an actinomycete isolated from desert soil.

Abstract

A novel actinomycete, designated strain XZHG99T, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China. Its taxonomic position was determined by a polyphasic approach. The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces: branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analysis indicated that strain XZHG99T showed high similarity to Streptomyces albiflavescensm20T (98.42 %) and Streptomyces krungchingensis KC-035T (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree. Based on comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain XZHG99T can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces dengpaensis sp. nov. is proposed. The type strain is XZHG99T (=CGMCC 4.7472T=KCTC 49090T).