Streptomyces aridus sp. nov., isolated from a high altitude Atacama Desert soil and emended description of Streptomyces noboritoensis Isono et al. 1957

Hamidah Idris,Juan A. Asenjo,Alan T. Bull,Maria del Carmen Montero-Calasanz,Jean Franco Castro,Imen Nouioui,Michael Goodfellow,David P. Labeda

doi:10.1007/s10482-017-0838-2

Abstract

A polyphasic study was undertaken to determine the taxonomic status of a Streptomyces strain which had been isolated from a high altitude Atacama Desert soil and shown to have bioactive properties. The strain, isolate H9T, was found to have chemotaxonomic, cultural and morphological properties that place it in the genus Streptomyces. 16S rRNA gene sequence analyses showed that the isolate forms a distinct branch at the periphery of a well-delineated subclade in the Streptomyces 16S rRNA gene tree together with the type strains of Streptomyces crystallinus, Streptomyces melanogenes and Streptomyces noboritoensis. Multi-locus sequence analysis (MLSA) based on five house-keeping gene alleles showed that isolate H9T is closely related to the latter two type strains and to Streptomyces polyantibioticus NRRL B-24448T. The isolate was distinguished readily from the type strains of S. melanogenes, S. noboritoensis and S. polyantibioticus using a combination of phenotypic properties. Consequently, the isolate is considered to represent a new species of Streptomyces for which the name Streptomyces aridus sp. nov. is proposed; the type strain is H9T (=NCIMB 14965T=NRRL B65268T). In addition, the MLSA and phenotypic data show that the S. melanogenes and S. noboritoensis type strains belong to a single species, it is proposed that S. melanogenes be recognised as a heterotypic synonym of S. noboritoensis for which an emended description is given.

Highlights

Streptomycetes remain a unique source of novel pharmaceutically important products with antibacterial, anti-inflammatory and antitumor activities (Berdy 2005; Demain 2014; Barka et al 2016), the continued interest in these organisms as a source of new specialised metabolites (Goodfellow and Fiedler 2010; Chaudhary et al 2013)
Previous work from our group has shown that novel Streptomyces species abound in arid Atacama Desert soils (Okoro et al 2009; Busarakam 2014; Busarakam et al 2014), some of which synthesise new specialised metabolites with encouraging bioactivities (Bull et al 2016), thereby underpinning the premise that extreme environmental conditions promote unique actinobacterial diversity which is the basis of novel chemistry (Bull and Stach 2007; Bull 2011; Gomez-Escribano et al 2015)
The results of a polyphasic taxonomic study showed that isolate H9T belongs to a novel Streptomyces species for which we propose the name Streptomyces aridus sp. nov

Summary

Introduction

Streptomycetes remain a unique source of novel pharmaceutically important products with antibacterial, anti-inflammatory and antitumor activities (Berdy 2005; Demain 2014; Barka et al 2016), the continued interest in these organisms as a source of new specialised metabolites (Goodfellow and Fiedler 2010; Chaudhary et al 2013). Multi-locus sequence analysis (MLSA) based on five house-keeping gene alleles showed that isolate H9T is closely related to the latter two type strains and to Streptomyces polyantibioticus NRRL B-24448T.

Objectives

Results

Conclusion