Streptomyces arboris sp. nov., isolated from Populus euphratica wetland soil.

Abstract

A novel actinobacterial strain (TRM 68085T) was isolated from soil of Populus euphratica wetland. A polyphasic approach was used to study the taxonomy of TRM 68085T and the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085T showed the highest similarity value to Streptomyces capitiformicae 1H-SSA4T (98.6 %), and phylogenetically clustered with Streptomyces kanasensis ZX01T(97.5 %) and Streptomyces ipomoeae NBRC 13050T (97.4 %). The genomic DNA G+C content of strain TRM 68085T based on the genome sequence was 71.4 mol%. The levels of DNA-DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours, S. capitiformicae 1H-SSA4T, S. kanasensis ZX01T and S. ipomoeae NBRC 13050T, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085T possessed MK-9(H6) and MK-9(H8) as the predominant menaquinones. ll-Diaminopimelic acid and a small amount of meso-diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C16 : 0 (25.4 %) and iso-C16 : 0 (18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085T represents a novel species of the genus Streptomyces, for which the name Streptomyces arboris sp. nov. is proposed. The type strain is TRM 68085T (=CCTCC AA2019031T=LMG 31492T).