Streptococcus mutans and Lactobacillus Species Infection in Obese and Non-Obese School Children in Accra, Ghana

Abstract

Introduction Several bacteria colonize the oral cavity. Some are involved in oral diseases including dental caries. Dental caries is one of the commonest oral problems in children [1]. Acid producing oral bacteria such as Streptococcus mutans and Lactobacillus species are the major bacteria associated with caries [2]. For a long time it was thought that the presence of these bacteria alone may lead to dental caries but later studies have demonstrated that there were cases where caries occurred even in the absence of these bacteria [3,4]. Some of these other bacteria include Streptococcus sanguinis, Streptococcus oralis, Neisseria subflava. However, when Streptococcus mutans and Lactobacillus species dominate the plaque caries occurs faster [2,3]. Colonization by some of the oral bacterial starts even before the eruption of teeth in the mouth of children [5]. These bacteria are thought to be transmitted from mother to child [6-9]. Background: Streptococcus mutans and Lactobacillus species are major bacteria associated with dental caries. In the presence of fermentable sugars and poor oral hygiene these bacteria increase risk of caries. Some studies have associated obesity with dental caries while others did not. High sugar snacks are risks for both obesity and dental caries. Citation: Ndanu TA, Aryeetey R, Sackeyfio J, Otoo G, Lartey A, et al. (2015) Streptococcus Mutans and Lactobacillus Species Infection in Obese and Non-Obese School Children in Accra, Ghana. J Obes Overweig 1(1): 101 Objectives: This study aimed at determining Streptococcus mutans and Lactobacillus species infection and its relationship with dental caries among obese and non-obese children. Methods: Oral plaque was taken from 75 each of obese and non-obese children from private schools in Accra, Ghana. The plaque was pre-enriched in thioglycollate broth for 48 hours at 37 °C. Streptococcus mutans and Lactobacillus species were recovered after sub-culturing the broths onto Mitis Salivarius Bacitracin (MSB) and Man Rogosa Sharpe (MRS) selective plates, respectively, and incubation at 37 °C under anaerobic conditions. Bacteria isolates on the selective plates were identified using Gram morphology and catalase activity. Results: Streptococcus mutans was isolated in 51 (33.6%) of all 150 children. The prevalence of Streptococcus mutans was significantly higher in the obese children 31 (41.3%) than the non-obese 20 (26.0%), p=0.033. Recovery of Lactobacillus species was low 2 (1.3%). Caries prevalence was similar in the obese 14.9% and non-obese 15.1%. There was no significant association between Streptococcus mutans infection and the dental caries. Overall Silness and LOe Plaque Index of 1.1 ± 0.5 indicate good oral hygiene status for the children. Conclusion: Obese school children had higher oral Streptococcus mutans infections than non-obese children but this was not significantly associated with dental caries prevalence.