Abstract
Biochemical and molecular genetic studies were performed on a novel Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from tonsil samples of two Iberian ibexes. The micro-organism was identified as a streptococcal species based on its cellular, morphological and biochemical characteristics. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus, but the organism did not correspond to any species of this genus. The nearest phylogenetic relative of the unknown coccus from ibex was Streptococcus porci 2923-03T (96.6 % 16S rRNA gene sequence similarity). Analysis based on rpoB and sodA gene sequences revealed sequence similarity values lower than 86.0 and 83.8 %, respectively, from the type strains of recognized Streptococcus species. The novel bacterial isolate was distinguished from Streptococcus porci and other Streptococcus species using biochemical tests. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as representing a novel species of the genus Streptococcus, for which the name Streptococcus caprae sp. nov. is proposed. The type strain is DICM07-02790-1CT ( = CECT 8872T = CCUG 67170T).
Highlights
From two animals that were hunted on the same day and at the same location (National Game Reserve of Puertos de TortosaBeceite, north-eastern Spain)
Sequence searches revealed that the unknown cocci were members of the genus Streptococcus, being related most closely to Streptococcus porci 2923-03T (96.6 % 16S rRNA gene sequence similarity)
Phylogenetic trees were reconstructed according to three different algorithms: neighbour-joining (Saitou & Nei, 1987) using the programs SeqTools and TreeView (Page, 1996; Rasmussen, 2002), maximum-parsimony using the software package MEGA version 4 (Kumar et al, 2004) and maximum-likelihood using the PHYML software (Guindon & Gascuel, 2003)
Summary
From two animals that were hunted on the same day and at the same location (National Game Reserve of Puertos de TortosaBeceite, north-eastern Spain). Phenotypic and phylogenetic characterization of both isolates were carried out and, based on the results, a novel species of the genus Streptococcus is proposed. Sequence searches revealed that the unknown cocci were members of the genus Streptococcus, being related most closely to Streptococcus porci 2923-03T (96.6 % 16S rRNA gene sequence similarity).
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