Abstract
Electron paramagnetic resonance (EPR) in conjunction with a slow-tumbling simulation was utilized for examining the effect of diluted detergent-treated stratum corneum (SC) lipid structure. SC from the back of hairless mouse (HOS:HR-1) was stripped consecutively from one to three times using a glass plate coated with a cyanoacrylate resin. Aliphatic spin probe, 5-doxylstearic acid (5-DSA), was used to evaluate the SC ordering. The EPR spectrum of 5-DSA incorporated in the SC demonstrated a characteristic peak for the first strip. A slow-tumbling simulation for 5-DSA showed slight differences in ordering values (S 0) of the SC for the control and detergent-treated SC. The EPR spectra showed that the characteristic mobile component was eliminated. The values were 0.15 and 0.32, respectively. Thus, the EPR method along with the simulation analysis revealed the differences in ordering of the detergent-treated SC.
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