Strategy for improvement of doubled haploid production in maize

Abstract

In the present study the applicability of a self-constructed doubled haploid line (DH 105) in the in vitro breeding of maize was evaluated. This line, which contained only 50% exotic (Chinese) germplasm, could be used to transmit in vitro androgenic ability into non-responsive breeding materials. F1 hybrids resulting from single crosses between the moderately responsive line DH 105 and recalcitrant genotypes with high breeding value showed a considerable heterosis effect in their androgenic responses. Most of the hybrids had favourable morphological and agronomic characters on the basis of “per se” evaluation. The data of the experiments showed that these F1 hybrid plants could be successfully used as anther donors, since numerous fertile DH plants were developed from their anther cultures. By the use of this in vitro breeding strategy the genetic variability can be widened and the effectiveness of inbred line production might be improved.