Abstract
ObjectiveTo evaluate and compare the distribution and density of primordial follicles within a whole sheep ovary and to gain insight into how to overcome the impact of natural follicular heterogeneity on the experimental results.DesignHistological study.SettingAcademic research center.AnimalsFive- to nine-month-old ewes.InterventionsFreshly sampled whole sheep ovaries were collected and prepared for histological analysis.Main Outcome Measure(s)The follicular densities and distributions were determined for hematoxylin and eosin sections. A mathematical model was derived based on the follicle counts and Monte-Carlo simulations.ResultsHeterogeneous distributions and densities of primordial follicles were identified 1) for distinct areas of the same ovarian cortex, 2) between the ovaries of the same animal and 3) across different ewes. A mathematical model based on the analysis of 37,153 primordial follicles from 8 different ovaries facilitated the estimation of the number of cortical biopsies and sections that had to be analyzed to overcome such heterogeneity.ConclusionThe influence of physiological follicular heterogeneity on experimental and clinical results can be overcome when a definite number of cortical pieces and sections are taken into consideration.
Highlights
A mathematical model based on the analysis of 37,153 primordial follicles from 8 different ovaries facilitated the estimation of the number of cortical biopsies and sections that had to be analyzed to overcome such heterogeneity
The influence of physiological follicular heterogeneity on experimental and clinical results can be overcome when a definite number of cortical pieces and sections are taken into consideration
Ovarian cryopreservation is a promising technique for fertility preservation in young women with cancer prior to sterilization by chemotherapy or radiotherapy
Summary
Ovarian cryopreservation is a promising technique for fertility preservation in young women with cancer prior to sterilization by chemotherapy or radiotherapy. This technique creates the hope of restoring gonadal function in iatrogenically sterilized patients upon autografting [1,2]. Fifteen years of clinical advances in ovarian tissue cryopreservation and cryobanking demonstrate that the procedure is safe, easy and promising. The pregnancy rate after the autografting of cryopreserved tissue to orthotopic sites is estimated to be approximately 30%, the precise denominator is currently unknown [4]. Approximately 24 infants have been born worldwide using this procedure [5]
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