STRATEGIES FOR IMPROVEMENT FOR CHARACTERIZATION AND QUALITY OF BT COTTON SEEDS

Abstract

The study highlights diverse methodologies employed for seed quality assessment and characterization, including genetic, biochemical, and physiological analyses. In our present study, PCR and ELISA based techniques was adopted to identify the specific Cry1Ac gene and proteins in MECH 162 and RCH2 transgenic cotton plants. MECH 162 of 21.9ug/g and RCH2 of 17.37ug/g of seed protein was extracted. PCR analysis confirmed that gene specific CRY1Ac F/R primer as amplicon size of 550 bp for Bt gene, 600bp for NptII gene and 200bp for 35s promoter gene. The ELISA method was used for the validation of the developed assay, also ELISA needed simple equipment and took less time. Insights into the integration of these strategies into breeding programs are provided, emphasizing their crucial role in ensuring the production of high-quality Bt cotton seeds with improved agronomic performance and yield potential.