Abstract
ABSTRACTThe present study focused on the enhancement of hydrogen yield by using mixed culture (sewage sludge and/or slaughterhouse sludge) from starch effluent. The process parameters such as pH, temperature and substrate concentration were studied. The maximum hydrogen yield of 5.5 mol/kg chemical oxygen demand (COD) was obtained in the initial optimization study in the dark fermentation at pH, temperature and substrate concentration of 6.5, 55 °C and 15 g/L, respectively. To enhance the hydrogen production the two different strategies were applied at optimum conditions such as (i) a combination of two mixed cultures (anaerobic sewage sludge and slaughterhouse sludge), and (ii) addition of crude amylase to the hydrogen production reactor while using anaerobic mixed sludge as inoculum and starch effluent as substrate. These two strategies gave the maximum hydrogen yield of 8.1 and 7.1 mol/kg COD, respectively.
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