Abstract
Modification of structural components, especially the cell wall, through adequate pretreatment strategies is critical to the bioconversion efficiency of algal biomass to biorefinery products. Over the years, several physical, physicochemical, chemical and green pretreatment methods have been developed to achieve maximum productivity of desirable by-products to sustain a circular bioeconomy. The effectiveness of the pretreatment methods is however, species specific due to diversity in the innate nature of the microalgal cell wall. This review provides a comprehensive overview of the most notable and promising pretreatment strategies for several microalgae species. Methods including the application of stress, ultrasound, electromagnetic fields, pressure, heat as well as chemical solvents (ionic liquids, supercritical fluids, deep eutectic solvents etc.) have been detailed and analyzed. Enzyme and hydrolytic microorganism based green pretreatment methods have also been reviewed. Metabolic engineering of microorganisms for product specificity and lower inhibitors can be a future breakthrough in microalgal pretreatment.
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