Strategic development to stabilize bioactive diallyl thiosulfinate by pH responsive non ionic micelle carrier system

Abstract

Diallyl thiosulfinate, extracted from the crushed garlic (Allium sativum) biomass is a crucial and principal bioactive compound having multiple noteworthy therapeutic properties. However, the unstable nature of diallyl thiosulfinate (allicin) is a major concern for its wide application in pharmaceutical products. Hence, suitable pH responsive micelle carrier systems were formulated using Polysorbate 20 and 80 for the enhancement of allicin’s stability while maintaining its concentration and bioactivity. The entrapment of allicin in the nonionic polyorbate micelle showed a significant reduction in its degradation at 25 °C temperature. Polysorbate 80 based micelle carrier system showed better entrapment and stability compared to Polysorbate 20 based micelles. Further, the ANOVA study showed that the temperature and pH are significant controlling factors for allicin’s stability within micelle (p < 0.05). Moreover, acidic pH significantly assists the release of allicin from the micellized core. The pH responsive release of allicin from the micelle in acidic environment can be advantageous for the release of the drug in the human intestine. Moreover, it was ensured that the micellization process did not hinder the bioactiveness of allicin and expedient synergistic antibacterial property with other antibiotics. The present investigation amplifies the possibility of producing commercial synergistic antimicrobials for various pharmaceutical applications.