Strategic adjustment of copulatory plug size in a nematode.

Abstract

Copulatory plugs (CP) are substances produced during copulation that block the genital openings of the female. In several species of Nematoda, males produce CP that are thought to impede female remating and thus sperm competition. The relatively large size of the CP in several nematodes, and its evolutionary loss in self-fertilizing populations of Caenorhabditis elegans, suggests that CP are costly to produce. If CP production is costly, the application of basic concepts of strategic ejaculation theory suggests a modulated allocation of CP in response to sperm competition risk. This hypothesis led us to predict that males perceiving a higher risk of sperm competition will produce larger CP. We tested these ideas with the entomopathogenic, gonochoristic nematode Rhabditis regina. Our first experiment provides evidence suggesting that production of CP is costly, because the size of CP is negatively affected by stressful conditions (high population density, small male adult size, and suboptimal food type). The results of our second experiment support the prediction that males adjust the size of CP to sperm competition risk: the average size of CP increased as the number of males competing for one female increased. Overall, our study supports the idea that in R. regina the production of CP is costly for males and that the size of the CP produced is influenced by sperm competition risk.