Abstract
BackgroundMicroRNAs (miRNAs) play crucial roles in varieties of cancers, particularly in tumorigenesis, progression, and migration. Dysregulation of miR-28 was reported to occur in various types of human malignancies. In humans, two different mature miRNA sequences are excised from opposite arms of the stem-loop pre-miR-28, hsa-miR-28-3p and hsamiR-28-5p. However, the expression and distinct role of miR-28-3p and miR-28-5p in nasopharyngeal carcinoma (NPC) remain undetermined.MethodsThe expressions of miR-28-3p/-5p in human NPC tissues were tested by quantitative real-time PCR. miR-28-3p/-5p were overexpressed by mimics and silenced by inhibitors. The roles of miR-28-3p/-5p in NPC development were studied using cultured HONE-1 cells.ResultsThe mRNA expression levels of miR-28-3p and -5p were significantly decreased in NPC tissues in comparison with adjacent normal tissues. Overexpression of miR-28-5p suppressed NPC cell proliferation and induced cell cycle arrest and apoptosis, while miR-28-3p promoted NPC cell migration and invasion. The miRNAs effected on different signal pathways: miR-28-5p altered expression of cyclin D1 and influenced the PI3K/AKT signaling pathway. In contrast, miR-28-3p downregulated Nm23-H1 and accelerated the process of EMT.ConclusionmiR-28-3p and -5p were both downregulated in NPC tissues but had distinct biological effects in NPC cells. They may serve as potential prognostic markers and therapeutic targets for NPC.
Highlights
MicroRNAs play crucial roles in varieties of cancers, in tumorigenesis, progression, and migration
We discovered that miR-28-3p and miR28-5p were both downregulated in nasopharyngeal carcinoma (NPC) tissues but had distinct biological effects in NPC cells. miR-28-5p inhibited NPC cell proliferation, and induced apoptosis and cell cycle arrest but had no effect on cell migration and invasion in vitro
We report that expression of miR-28-3p and miR-28-5p are downregulated in NPC tissues
Summary
MicroRNAs (miRNAs) play crucial roles in varieties of cancers, in tumorigenesis, progression, and migration. Nasopharyngeal carcinoma (NPC) is a highly malignant disease that originates in the nasopharynx epithelium. It has a high incidence in Southern China, Southeast Asia and North Africa [1]. MicroRNAs (miRNAs) are highly conserved, endogenous small noncoding RNAs with ~ 20 nucleotides, that disturb gene expression by binding to the 3′ untranslated region (3′UTR) of their target mRNAs [8]. It can regulate gene expression at the translational or. The function of miRNAs in NPC cells is still largely unexplored
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