Abstract
To contribute to our general understanding of the evolutionary forces that shape variation in genome sequences in nature, we have sequenced genomes from 50 isofemale lines and six pooled samples from populations of Drosophila melanogaster on three continents. Analysis of raw and reference-mapped reads indicates the quality of these genomic sequence data is very high. Comparison of the predicted and experimentally-determined Wolbachia infection status of these samples suggests that strain or sample swaps are unlikely to have occurred in the generation of these data. Genome sequences are freely available in the European Nucleotide Archive under accession ERP009059. Isofemale lines can be obtained from the Drosophila Species Stock Center.
Highlights
Whole genome shotgun sequences can be generated using short-read sequencing technology for most organisms
Hundreds of resequenced genomes exist for Drosophila melanogaster that can be used for population and genomic analysis in this model insect species (Lack et al, 2014)
To contribute to the worldwide sampling of population genomic data in D. melanogaster, we have sequenced genomes of multiple isofemale lines from three populations collected on different continents reported in Verspoor & Haddrill (2011): Montpellier, France (FR, n=20), Athens, Georgia, USA (GA, n=15) and Accra, Ghana (GH, n=15)
Summary
Whole genome shotgun sequences can be generated using short-read sequencing technology for most organisms. To contribute to the worldwide sampling of population genomic data in D. melanogaster, we have sequenced genomes of multiple isofemale lines from three populations collected on different continents reported in Verspoor & Haddrill (2011): Montpellier, France (FR, n=20), Athens, Georgia, USA (GA, n=15) and Accra, Ghana (GH, n=15). Strains sequenced here were chosen because isofemale lines exist in the Drosophila Species Stock Center and because their infection status for the Wolbachia pipientis bacterial endosymbiont had previously been determined (Verspoor & Haddrill, 2011).
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