Abstract
North Carolina and Bermuda isolates of the green seaweed Ulvaria oxysperma (Kuetz.) Bliding were grown in cross-gradient light-temperature culture to determine the effects of varied photon fluence rates, temperature and photoperiod on their growth and reproduction. Results indicated significant genetic-based variation between the two populations. Spheroplast were obtained from the vegetative thallus of the North Carolina isolate following enzymatic digestion with abalone gut extract and maceration. Single cell isolates developed new cell walls in 2–3 days and attached to nylon net substrate. Cultured isolates regenerated into juvenile plants with normal development and morphology. Results are discussed in terms of the suitability of this economically important species for land-based mariculture.
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