Abstract
Cellulases are multi-enzymatic complex proteins that catalyze the conversion of cellulose to glucose. Indigenous strain of Trichoderma viride FCBP-142 was selected to develop over-producer of cellulases and subjected to mutagenesis withultra violet (UV) and chemical ethyl methane sulfonate (EMS). Among 178 survivals after UV irradiation, bigger zones of clearing on agar plates appeared around 81 colonies of putative mutant strains of native fungus with maximum of 87 IU/ml by Tv-UV-5.6 strain in comparison to parental strain (53 IU/ml). For EMS treatment, the enzyme production by the most active mutant Tv-Ch-4.3 showed even higher-level of cellulase activity (122.66 IU/ml) in contrast to the UV and parental strains. Genetic relationships of stable mutants of T. viride were also analyzed with RAPD-PCR. Results obtained from the comparison between genotypes of T. viride exhibited differences in sizes and numbers of amplified fragments per primer for each isolate. The dendrogram showed that the genotypes Tv-Ch-4.3 and Tv-Ch-5.5 were distinctly classified into one category, while the two isolates (Tv-UV-5.6 and Tv-UV-5.9) of T. viride FCBP-142 (parental) have the nearest genetic relationship. Moreover, the five isolates from T. viride genotypes shared an average of 75 percent bands. Key words: Cellulase activity, Trichoderma viride, UV irradiation, EMS treatment, RAPD-PCR.
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