Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103.

C Subathra Devi,Anuj Saini,S Jemimah Naine,Shubham Rastogi,V Mohanasrinivasan

doi:10.1007/s13205-013-0186-5

C Subathra Devi, Anuj Saini + Show 3 more

Open Access

https://doi.org/10.1007/s13205-013-0186-5

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Journal: 3 Biotech	Publication Date: Dec 18, 2013
Citations: 26	License type: CC BY 2.0

Affiliation: Vellore Institute of Technology University

Abstract

In the present study, Saccharopolyspora erythraea MTCC 1103 was used for the enhanced production of erythromycin. To enhance the yield of erythromycin, effects of various parameters such as bagasse concentration, organic nitrogen source, inorganic nitrogen source, pH and temperature were analysed. It was found that bagasse can be used as an alternate carbon source in erythromycin production medium. Erythromycin production in the new formulation of bagasse based medium was found to be 512 mg/L which was 28 % higher than glucose based medium. Strain improvement was done by random UV-mutagenesis. When compared to wild type strain, mutant strain showed 40 % higher yield in production medium. Erythromycin potency assay and HPLC analysis were performed to confirm the presence of erythromycin in the partially purified samples. These optimized conditions could be used for the commercial production of this unique antibiotic which gave significant industrial perspectives.

Highlights

The discovery of novel antimicrobial compounds is currently the thirst area of research (Jemimah Naine et al 2012)
The primary aim of this work was to search for a cheap carbon source which is readily available to suffice the basic need of a microbe without compromising the quality of the final product, erythromycin
The results suggested that bagasse can be effectively used for production of erythromycin

Summary

Introduction

The discovery of novel antimicrobial compounds is currently the thirst area of research (Jemimah Naine et al 2012). The remarkable carbon source bagasse is a cheap and readily available substrate for the high yield of antibiotic It laid the foundation for the future course of action which was to optimize substrate (bagasse) concentration at standard temperature and pH for maximum erythromycin production. The strain improvement studies were carried out on the same MTCC 1103 strain so as to increase the production rate further, which has its immense value on the commercial scale. All these studies underwent had a sole purpose of reducing the cost of the primary antibiotic, erythromycin which is indispensable for the creation of future generation antibiotics like azithromycin

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Conclusion