Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse.

Naushad Ahmad Khan,Irfan Rahman,Isaac Kirubakaran Sundar

doi:10.14814/phy2.13579

Naushad Ahmad Khan, Irfan Rahman + Show 1 more

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https://doi.org/10.14814/phy2.13579

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Abstract

Waterpipe smoking is emerging as a form of tobacco smoking, but its lung health/risks is not known. It has been shown that different mouse strains show differences in susceptibility to tobacco smoke. However, the effect of waterpipe smoke (WPS) exposure and strain differences in susceptibility to oxidative and inflammatory responses is not known. Here, we showed acute WPS exposure induced oxidative stress and inflammatory response in C57BL/6J and BALB/cJ mouse strains. WPS exposure induced inflammatory cell influx (neutrophils and T‐lymphocytes) in bronchoalveolar lavage fluid (BAL fluid), which varied among mouse strains. Proinflammatory cytokines release differed among both the strains, but was significantly increased in C57BL/6J mice. Myeloperoxidase levels in BAL fluid were increased significantly in both the strains. Total reduced glutathione (GSH) level was decreased, whereas the level of oxidized or glutathione disulfide (GSSG) increased in lungs of both the strains. Similarly, the level of lipid peroxidation markers, 15‐isoprostane (plasma), malondialdehyde and 4‐hydroxy‐2‐nonenal (lung homogenates) were increased by WPS. Our data suggest that, oxidative stress and inflammatory responses are influenced by strain characteristics during acute WPS exposure. Overall, C57BL/6J mice showed more susceptibility to oxidative stress and inflammatory responses compared to BALB/cJ mice. Acute WPS mediated pulmonary toxicity is differentially regulated in different mouse strains.

Highlights

Waterpipe smoking involves the passage of charcoal-heated air through a perforated aluminum foil and across flavored tobacco (Javed et al 2017)
Our data showed that acute waterpipe smoke (WPS) exposure significantly increased the 4-HNE levels in both the strains of mice compared to air control (Fig. 5E)
We studied the molecular mechanism of susceptibility to acute pulmonary toxicity induced by WPS using C57BL/6J and BALB/cJ mice

Summary

Introduction

Waterpipe smoking ( known as hookah, shisha, hubble bubble, and narghila) involves the passage of charcoal-heated air through a perforated aluminum foil and across flavored tobacco (molasses) (Javed et al 2017). This generates smoke which bubbles through water before being inhaled by the users (Fig. 1). Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

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