Strabismus modifies intrinsic and inter-areal connections in cat area 18

Abstract

The development of long-range horizontal connections depends on visual experience. Previous experiments have shown that in area 17 of strabismic but not in normal cats, horizontal fibers preferentially connect cell groups driven by the same eye indicating that fibers between co-active neurons are selectively stabilized. To test whether this is a general organizing principle of intracortical long-range circuitry we extended our analyses to both intrinsic horizontal connections within area 18 and to inter-areal connections between areas 17 and 18. To this end, we visualized the functional architecture of area 18 by intrinsic signal imaging. Horizontal circuitry was labeled by injecting fluorescent latex microspheres into functionally identified domains. Additionally, domains sharing the same ocular dominance as the neurons at the injection sites were visualized by 2-deoxyglucose autoradiography to allow comprehensive labeling of functional domains in regions far from the injection sites. Quantitative analyses revealed that in strabismic cats, 72% of the retrogradely labeled neurons in area 18 and 68% of the neurons in area 17 were located in the same ocular dominance domains as the injection sites. In contrast, these numbers were 52% and 54% in normal animals. These data show that experience modifies both intrinsic connections within area 18 and inter-areal projections from area 17 to area 18 as has been previously described for intrinsic and callosal connections in area 17. This provides further evidence for the hypothesis that the correlation of activity is a major selection criterion for the stabilization of neuronal circuits during postnatal development.