Abstract
Pyrosequencing is a fast, real-time, non-electrophoretic sequencing-by-synthesis method that can be used to genotype short tandem repeat (STRs) markers. In this study, a total of 18 Y-chromosome and autosomal STRs have been successfully analyzed using Pyrosequencing technology. Several sequence variants were detected, demonstrating that additional information besides the fragment length can be provided in a forensic DNA investigation by these assays.
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