Abstract
Dentin provides a protective enclosure for genomic and mitochondrial DNA. In the present study, DNA was obtained from pulverized or ground teeth. The quality of the DNA extracted from the teeth of 70 unrelated individuals was tested in the context of assessing the allelic and genotypic frequencies of autosomal loci D19S216, D20S502 and D20S842, and calculating a number of parameters of population genetics and forensic interest. This study illustrates that teeth can be a convenient tissue to extract DNA from large numbers of individuals for population genetic studies as well as for forensic case work.
Highlights
A suitable approach in forensic and medical DNA typing is the use of genetic material from hard tissues including bones and teeth
We aimed to establish the usefulness of dental tissue as a source of DNA for genotyping the hypervariable short tandem repeat (STR) loci D19S216, D20S502 and D20S842, which are of medicinal, forensic and population genetic interest
The quantity of dentin material used for DNA extraction varied between 1.5 and 2.2 g
Summary
A suitable approach in forensic and medical DNA typing is the use of genetic material from hard tissues including bones and teeth. Dental specimens have been used as a source of DNA in forensic analysis with various degrees of success, depending on the type of polymorphic markers employed and the extraction method utilized (Trivedi et al, 2002, Yamamoto, 1996). STR loci are currently widely used in generating population databases for human DNA profiling (Rowold and Herrera, 2005a). STR polymorphisms are very important markers for human identification and phylogenetic studies due to their hypervariable nature (Rowold and Herrera, 2003b). We aimed to establish the usefulness of dental tissue as a source of DNA for genotyping the hypervariable STR loci D19S216, D20S502 and D20S842, which are of medicinal, forensic and population genetic interest
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
