Stored polyadenylated RNA and loss of vigour in germinating wheat embryos

Abstract

Loss of vigour in wheat seed is associated with lesions affecting the rate of disappearance of stored poly A + RNA (presumptive mRNA) in the germinating embryo when germination takes place at a sub-optimal temperature. During germination in the presence of α-amanitin and consequent of de novo polyA + RNA biosynthesis, the wheat embryo can degrade up to 70% of the stored poly A + RNA of the quiescent embryo before any significant reduction in the rate of protein biosynthesis in the embryo becomes apparent. It is possible that two subpopulations of poly A + RNA species exist in wheat embryos during early germination, one population being degraded rapidly upon rehydration of the embryo whilst the other population supports protein biosynthesis in the initial germination stages prior to degradation.