Abstract
Seed reserve mobilization is considered a post-germination process; however, seed storage protein mobilization occurs during germination. Thus, the knowledge of seed protein composition is important to understand various processes during germination, and it be associated with seed vigor. Therefore, this study aimed to characterize landrace genotypes of common bean (Phaseolus vulgaris) through fractionation of seed storage proteins during germination and to verify the association between seed protein composition and seed vigor. Genotypes of the highest (55 and 81) and lowest (23 and 50) physiological quality were selected. Protein content analysis, profiling, and characterization were performed by combining different hydration times. Mass spectrometry involving in-gel digestion and MALDI-ToF analysis was employed for the qualitative identification of proteins. Glutelin extraction detected the enzyme lipoxygenase in genotypes 55 and 81 alone. In plants, this enzyme may be involved in diverse physiological processes, including growth and development, pest resistance, senescence, and response to wounding. In the process of germination, lipoxygenase removes reactive oxygen species during reserve mobilization; therefore, lipoxygenase may be a candidate biochemical marker for high-vigor genotypes.
Highlights
IntroductionGermination involves various physiological processes that are sequentially initiated by water uptake (imbibition; D'Hooghe et al, 2019)
Many studies of bean seed proteomics have demonstrated the efficiency of different protein extraction methods to optimize the quality of separation and subsequent identification of proteins (Martínez et al, 2012)
Data are expressed as mg·100 mg-1 of cotyledons dry matter
Summary
Germination involves various physiological processes that are sequentially initiated by water uptake (imbibition; D'Hooghe et al, 2019). Seed hydration increases respiration and metabolism, allowing the mobilization of carbon and nitrogen reserves necessary to support germination and post-germination growth until autotrophy is reached (Anzala, Paven, Fournier, Rondeau, & Limami, 2006). Seed germination is a physiological and biochemical process that involves different cascades of signal transduction and gene expression regulation. The elucidation and subsequent availability of bean genome have enabled the rapid advancement of seed proteomics (Rossi, Valentim-Neto, Blank, Faria, & Arisi, 2017). Many studies of bean seed proteomics have demonstrated the efficiency of different protein extraction methods to optimize the quality of separation and subsequent identification of proteins (Martínez et al, 2012)
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