Abstract
BackgroundStomatin-like protein 2 (SLP-2, also known as STOML2) is a stomatin homologue of uncertain function. SLP-2 overexpression has been suggested to be associated with cancer progression, resulting in adverse clinical outcomes in patients. Our study aim to investigate SLP-2 expression in epithelial ovarian cancer cells and its correlation with patient survival.MethodsSLP-2 mRNA and protein expression levels were analysed in five epithelial ovarian cancer cell lines and normal ovarian epithelial cells using real-time PCR and western blotting analysis. SLP-2 expression was investigated in eight matched-pair samples of epithelial ovarian cancer and adjacent noncancerous tissues from the same patients. Using immunohistochemistry, we examined the protein expression of paraffin-embedded specimens from 140 patients with epithelial ovarian cancer, 20 cases with borderline ovarian tumours, 20 cases with benign ovarian tumours, and 20 cases with normal ovarian tissues. Statistical analyses were applied to evaluate the clinicopathological significance of SLP-2 expression.ResultsSLP-2 mRNA and protein expression levels were significantly up-regulated in epithelial ovarian cancer cell lines and cancer tissues compared with normal ovarian epithelial cells and adjacent noncancerous ovarian tissues. Immunohistochemistry analysis revealed that the relative overexpression of SLP-2 was detected in 73.6 % (103/140) of the epithelial ovarian cancer specimens, 45.0 % (9/20) of the borderline ovarian specimens, 30.0 % (6/20) of the benign ovarian specimens and none of the normal ovarian specimens. SLP-2 protein expression in epithelial ovarian cancer was significantly correlated with the tumour stage (P < 0.001). Epithelial ovarian cancer patients with higher SLP-2 protein expression levels had shorter progress free survival and overall survival times compared to patients with lower SLP-2 protein expression levels. Multivariate analyses showed that SLP-2 expression levels were an independent prognostic factor for survival in epithelial ovarian cancer patients.ConclusionsSLP-2 mRNA and proteins were overexpressed in epithelial ovarian cancer tissues. SLP-2 protein overexpression was associated with advanced stage disease. Patients with higher SLP-2 protein expression had shorter progress free survival and poor overall survival times. Thus, SLP-2 protein expression was an independent prognostic factor for patients with epithelial ovarian cancer.
Highlights
Stomatin-like protein 2 (SLP-2, known as stomatin-like protein 2 (STOML2)) is a stomatin homologue of uncertain function
The SLP-2 protein was highly expressed in the epithelial ovarian cancer cell lines and only weakly expressed in the normal ovarian surface epithelial (NOSE) cells (Fig. 1b)
The SLP-2 mRNA and protein were overexpressed in epithelial ovarian cancer tissues To investigate the SLP-2 mRNA and protein expression levels in human epithelial ovarian cancer tissues, we used real-time RT-PCR and western blotting to analyse eight matched pairs of epithelial ovarian cancer specimens (T) and adjacent noncancerous tissue samples (ANT)
Summary
Stomatin-like protein 2 (SLP-2, known as STOML2) is a stomatin homologue of uncertain function. SLP-2 overexpression has been suggested to be associated with cancer progression, resulting in adverse clinical outcomes in patients. Our study aim to investigate SLP-2 expression in epithelial ovarian cancer cells and its correlation with patient survival. Despite progress in the development of new therapeutic methods, the 5-year survival rate of epithelial ovarian cancer patients has remained at approximately 30 % [3]. Transecting antisense SLP-2 into the oesophageal squamous cell carcinoma cell line TE12 reduced cell growth and adhesion These results suggested that SLP-2 was a potential oncogene [7, 8]. The expression status of SLP-2 and its clinical significance in epithelial ovarian cancer remain unclear. We investigated the protein and mRNA expression levels of SLP-2 in ovarian cancer tissues using immunohistochemistry, western blotting, and RT-PCR to analyse the potential clinical significance of SLP-2 expression
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