Abstract
Epidermal strips from either well-watered or water-stressed plants of Commelina communis L. were subjected to a range of ABA concentrations (10~6-10~3 mol m-3) in the presence (330 parts 10~6 in air) or virtual absence (3 parts 10 6 in air) of C02. The stomatal response to C02 was greater in epidermis from water-stressed plants, although there was a distinct C02 response in epidermis from well-watered plants. Additions of ABA via the incubation medium had little effect on the relative C02 response. Stomata responded to ABA both in the presence and virtual absence of C02, but the relative response to ABA was greatest in the high C02 treatment. When well-watered plants were sprayed with a 10 ' mol m~3 ABA solution 1 d prior to use, the stomatal response of detached epidermis to both C02 and ABA was very similar to that of epidermis detached from water-stressed leaves. It is hypothesized that a prolonged exposure to ABA is necessary before there is any modification of the C02 response of stomata.
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