Abstract
In vitro culture conditions have been found to adversely affect the number and morphology of stomata in Wrightia tomentosa. Acclimatized or in vivo hardened plants showed elliptical stomata in comparison to the stomata of leaves obtained from in vitro grown plants, which showed wide open apparatus. In vitro leaves showed higher stomatal percentage with 29.4% malformed stomata as compared to the other phases of micropropagation. The water loss was found to be more in detached leaves after a period of 3 h at room temperature in in vitro cultures as compared to the plantlets undergoing different phases of hardening and acclimatization. The present study emphasizes the importance of stomatal characteristics for successful acclimatization of plants to ex vitro environment.
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