Abstract
We report on the measurement and analysis of the polarization state of second harmonic signals generated by starch granules, using a four-channel photon counting based Stokes-polarimeter. Various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), and anisotropy are extracted from the 2D second harmonic Stokes images of starch granules. The concentric shell structure of a starch granule forms a natural photonic crystal structure. By integration over all the solid angle, it will allow very similar SHG quantum efficiency regardless of the angle or the states of incident polarization. Given type I phase matching and the concentric shell structure of a starch granule, one can easily infer the polarization states of the input beam from the resulting SH micrograph.
Highlights
Nonlinear microscopy techniques exploiting, for example, second and third harmonic generation (SHG and THG) [1], two-photon excitation fluorescence (TPEF) [2] and coherent anti-Stokes Raman scattering (CARS) [3] are widely used in cellular and tissue imaging because they allow high molecular contrast, enabling characterization of subcellular details
We report on the measurement and analysis of the polarization state of second harmonic signals generated by starch granules, using a fourchannel photon counting based Stokes-polarimeter
Various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), and anisotropy are extracted from the 2D second harmonic Stokes images of starch granules
Summary
For example, second and third harmonic generation (SHG and THG) [1], two-photon excitation fluorescence (TPEF) [2] and coherent anti-Stokes Raman scattering (CARS) [3] are widely used in cellular and tissue imaging because they allow high molecular contrast, enabling characterization of subcellular details. Combination of SHG and CARS microscopy has only recently, for instance, been used to investigate the internal structural and chemical information of starch granules [10]. It has, been demonstrated that there is a significant relationship between polarization-resolved SHG signals and the crystalline structure of different types of starch grains [11,12,13].
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