Stochastic processes dominated the soil bacterial community assemblages along an altitudinal gradient in boreal forests

Abstract

Soil bacteria, as integral components of the soil microbial community, play pivotal roles in biogeochemical cycles and ecosystem functions in boreal forests. The altitudinal patterns of soil bacteria have been widely reported, but their community assembly is uncertain. Here, we investigated the soil bacterial community attributes (diversity, taxonomic and functional composition, and bacterial interactions) and ecological processes associated with community assembly on Mt. Oakley in the northern Greater Khingan Mountains via Illumina MiSeq sequencing and functional annotation tools. The alpha diversity indices of the soil bacteria exhibited a progressively decreasing trend with increasing altitude across seasons. The relative abundance of the dominant bacterial taxa was more sensitive to altitude than to season. More complex (more nodes and links) bacterial interactions were detected at the lowest and highest altitudinal sites, as well as in September. The variations in the taxonomic and functional compositions of the soil bacteria induced by altitude were mainly driven by the variations in soil pH and extracellular enzyme activities. Stochastic (dispersal limitation and drift) processes largely controlled the soil bacterial community assemblages across spatiotemporal scales. The community assemblages of soil bacteria were affected by altitude-induced changes in the taxonomic composition and functional groups. Collectively, our results have significant implications for understanding bacterial biogeography and community assembly mechanisms along altitudinal gradients in boreal forest ecosystems.