Stochastic Processes Dominate Soil Microbial Community Assembly during the Restoration of Degraded Karst Forests

Abstract

The mechanisms underpinning the soil microbial community assembly are important, particularly in the fragile karst forest ecosystem. Despite such significance, relevant topics remain limited. We investigated a typical karst area, the Maolan National Nature Reserve in China. For this purpose, 30 forest dynamics plots were established on three restoration gradients in degraded karst forests, namely shrub, pioneer tree, and climax communities. Using vegetation surveys, we explored the diversity patterns, driving factors, and community assembly of the soil microbial communities during the restoration of degraded karst forest ecosystems. In addition, the soil physicochemical properties and macrogenomic sequencing data were examined. One-way analysis of variance and principal coordinates analysis showed no significant changes in soil microbial α-diversity during restoration, and the opposite pattern was observed for β-diversity. Variation partitioning analysis revealed that the combined effect of both soil microbial β-diversity and soil was significant (28% and 32% for bacteria and fungi, respectively). Pearson correlation analyses showed that plant species diversity and soil multifunctionality correlated significantly with soil microbial β-diversity. In contrast, the direct effect of plants was smaller (2% and 3% for bacteria and fungi, respectively). According to the dispersal–niche continuum index, stochastic processes were responsible for the assembly of the bacterial and fungal soil microbial communities. During restoration, the dominant influence of stochastic effects on the assembly of bacterial communities intensified. In contrast, the reverse tendency was observed in soil fungi. The investigation of the diversity pattern of soil microbial communities and their assembly can provide theoretical references for the restoration of degraded ecosystems.