Abstract
All known methods for solid-phase synthesis of molecular arrays exploit positioning techniques to deposit monomers on a substrate preferably high densely. In this paper, stochastic patterning of molecule spots (250 000 spots monomers/cm2) via random allocation of the microbeads on a microstructured glass is presented. The size and shape of the microbeads and the microcavities are selected in such a way so that only one microbead can fit into the respective microcavity. Each microbead can be loaded with a certain type of molecule e.g. amino acids and is brought in the microcavities stochastically. Applying solvent vapor and heating the substrate, the molecules are released from the microbeads and coupled to the functionalized substrate. To differentiate between the microbeads carrying different molecules, quantum dot labels are preliminary introduced into the microbeads. Fluorescence imaging and subsequent data analysis enable decoding of the molecule deposition patterns. After the coupling step is completed, the microbeads are mechanically removed from the microwells. The composition of the monomer microbeads, their deposition and the conditions of the monomer extraction are studied. The stochastic monomer patterning may be used to design novel molecular arrays.
Highlights
All known methods for solid-phase synthesis of molecular arrays exploit positioning techniques to deposit monomers on a substrate preferably high densely
We describe particles loaded with molecules such as amino acids that may enable the realization of these three principles for in situ synthesis of high-density molecular arrays
We focus on assembly of microparticles on the micro-structured surfaces, on the extraction of amino acids from these particles and on deriving the allocation of the monomers using quantum dot (QD) labels of the particles
Summary
All known methods for solid-phase synthesis of molecular arrays exploit positioning techniques to deposit monomers on a substrate preferably high densely. A set of microbeads labeled with individual types of QDs with emission maximum wavelengths ranging between 490 nm and 610 nm were manufactured and scanned in the fluorescence channels defined in the previous step. After random deposition of the microbeads on the flat functionalized substrate, they were imaged in the fluorescence channel 580/14 corresponding to the emission maximum of the QD labels (Fig. 6a).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
