Abstract
SEVERAL methods have been used to study neurohumoral release within the mammalian brain. These include the use of collecting cups affixed to the cortical surface1–5, ventricular cannulae for the perfusion of cerebrospinal fluid pathways6–8 and the push–pull cannula9. With the last device, perfusion fluid is passed through the inner tube of a concentric dual-barrelled cannula and is recovered by siphoning through the outer barrel. Because it makes possible perfusion of small regions of cerebral tissue, even in the depths of brain, the push–pull cannula has been widely used to investigate the spontaneous and stimulus-induced release of acetylcholine2,10–17.
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