Stimuli responsive nano-composite co-delivery of Doxorubicin and ciprofloxacin using HPLC-UV combined spectroscopy methods

Abstract

This work has been done to present a kind of stimuli-responsive drug nano-carrier for embedding and consequently delivering combinational pharmaceutical agents including Doxorubicin (DOX, anticancer) and Ciprofloxacin (Cipro, antibiotic) into or in close vicinity of ailment for enhancing therapeutic effects of drugs and reducing their side effects. Applied biocompatible magnetics drug carriers were synthesized through in-situ polymerization techniques. Characterization techniques reveal that the suggested nano-carrier had a well-defined spherical shape with the pore size of 10–30 nm and typical superparamagnetic features. Synthesized nano-carriers were used for loading drugs with the percentage of 53.90% and 82.32% for DOX and Cipro, respectively. On the other hand, nano-carriers were demonstrated as a kind of promising drug delivery system for in-vitro breast cancer investigations. Cumulative release profile of drugs was obtained at different pH values using methods of Ion-mobility spectrometry (IMS) and HPLC-UV to navigate magnetic polyurethane nano-composite kinetic mechanisms through different mathematical models. The results showed that the nano-carrier exhibited a smart controlled release profile following Higuchi model with the highest correlation coefficient. In-vitro Cytotoxicity of nano-composite was tracked on MCF-7 cell lines using methyl thiazole tetrazolium (MTT) viability assay which shows nano-carriers have no toxicity for intelligent targeted drug delivery.