Abstract
The effects of transferrin on the proliferation kinetics of these cells from day-14 lower first molars, cultured for 2–6 days in a chemically defined medium supplemented with 5 and 50 μg/ml of human diferric transferrin, were studied. Transferrin stimulated the mitotic and [ 3H]-thymidine labelling indices. These data were correlated with immunolocalization of the transferrin receptor using indirect immunofluorescence and specific monoclonal antibodies. The presence of specific transferrin receptors in pre-odontoblasts and pre-ameloblasts, and in ameloblasts of older teeth (day-18 to day-21), was also assessed by indirect immunofluorescence and binding experiments using iodinated transferrin.
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