Stimulation of thyroid function by several pituitary hormones results in an increase in plasma thyroxine and reverse triiodothyronine in tilapia (Tilapia nilotica)

Abstract

In this study, intravenous injection of several doses of porcine follicle stimulating hormone (pFSH: 0.002, 0.01, 0.05, and 0.5 micrograms/g body wt), bovine TSH (bTSH: 0.5 micrograms/g body wt), and ovine growth hormone (oGH: 0.04, 0.02, and 0.4 microgram/g body wt) stimulated an increase in plasma thyroxine (T4) and reverse triiodothyronine (rT3) in tilapia. This effect occurred in a dose-dependent manner. pFSH was the most potent in stimulating thyroid function. The dose of 0.002 microgram pFSH/g body wt increased plasma levels of T4 over control levels (2.59 +/- 0.16 ng/ml) about 2.5-fold within 4 hr, whereas a concentration of 0.5 micrograms/g body wt caused a great and prolonged increase of T4 and rT3 levels. Control levels (2.59 +/- 0.16 ng/ml for T4 and 40.37 +/- 8.60 pg/ml for rT3) were increased 19- and 22-fold respectively, over 24 hr. An increase of T4 and rT3 levels occurred also after injection of total hypophyseal extract and Con A II glycoprotein fraction of a tilapia pituitary homogenate, whereas the protein fraction failed to alter plasma concentrations of T4 and rT3. rT3 levels were also significantly increased at 2 hr, but not at 1 hr, after injection of T4. Basal T3 levels (1.90 +/- 0.22 ng/ml) were reduced by half over 24 hr in all experiments. These results suggest the existence, in tilapia, of a 5-D pathway deiodination of T4 which is pituitary independent. Stimulation of T4 release is always followed by an increase in plasma rT3 levels.