Abstract

[3H]thymidine incorporation during culture of thyroid follicles isolated from porcine glands (Schatz et al. 1983, 1986) can be taken for estimating thyroid growth stimulating immunoglobulins (TGI) (Drexhage et al. 1980; Chiovato et al. 1983; Valente et al. 1983). In this study we wanted to characterize our assay system in order to answer the question whether TSH itself was responsible for stimulation of [3H]thymidine incorporation observed when using TSH as standard in our experiments. Furthermore, the effect of TSH and epidermal growth factor (EGF) (Eggo et al. 1983; Westermark et al. 1985) should be tested both without and with anti-TSH serum or anti-EGF serum present in the medium.

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