Abstract
Abstract Resistance to antibody-complement- (Ab-C) mediated killing of guinea pig hepatoma cells (line-1 and line-10) correlates with the ability of the cells to synthesize lipids, but not DNA, RNA, protein, or complex carbohydrate. We therefore studied the synthesis and release of macromolecules (especially lipids) by tumor cells under attack by Ab plus C. Ab-sensitized tumor cells (TA) incorporated increased amounts of acetate, glycerol, or fatty acids into complex lipids compared to control cells within 5 to 10 min after the addition of guinea pig C (GPC) to the cells (TAC). TA, TC, or TAΔC (Ab plus heat-inactivated GPC) did not show this effect. No enhancement of DNA, RNA, or protein synthesis in TAC as compared with control cells was noted. Line-1 cells, which are killed by GPC when sensitized with excess anti-Forssman Ab (TAxs), demonstrated enhanced lipid synthesis within 1 to 3 min after the addition of GPC to TAxs, before measurable killing of the cells had occurred.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
