Abstract
Cessation of shoot elongation in seedlings of Salix pentandra L. is induced by short photoperiod. Gibbereliin A9 (GA9) applied either to the apical bud or injected into a mature leaf, induced shoot elongation under a short photoperiod of 12 h, and GA9 could completely substitute for a transfer to a long photoperiod. When [3H]GA9 or [2H2]GA9 was injected into a leaf, no [3H]GA9 was detected in the elongating apex and only traces of [3H]GA9 were found in the shoot above the treated leaf. By the use of gas chromatography‐mass spectrometry (GC‐MS), [2H2]GA20 was identified as the main metabolite of [2H2]GA9 in both the shoot and the treated leaf. In addition, [2H2]GA1 and [2H2]GA29 were also identified as metabolites of [2H2]GA9. These results are consistent with the hypothesis that exogenous GA, promotes shoot elongation in Salix through its metabolism to GA20 and GA,.
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