Abstract
Abstract In vitro-derived shoots of ‘Improved Blaze’ rose (Rosa hybrida L.) were used to investigate the cause of improved root initiation obtained by lowering the concentration of the Murashige and Skoog (MS) salt formulation in the nutrient medium. The number and length of roots per explant increased as the concentration of total nitrogen in the MS salt formulation was reduced from 60 to 7.5 mm. There was no effect on rooting with as much as a 16-fold reduction in the concentration of the remaining MS salts from that of the normal MS formulation, when total nitrogen was kept at a constant 7.5 mm. When nitrogen was maintained at 7.5 mm and the concentrations of the remaining salts were maintained at 1/2 times that of the MS salt formulation, rooting was unaffected by raising the total solute concentration with NaCl to that when the medium contained the full MS nitrogen salt complement. These results show that lowering the total mineral salt level in the nutrient medium provides a more favorable nitrogen salts concentration for rhizogenesis than that provided by the MS salt formulation.
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