Abstract
The effect of different elicitors and the precursor tryptamine on reserpine production in whole plant cultures of Rauwolfia serpentina was examined. The elicitors include dimethyl sulphoxide (DMSO), Abscisic acid (ABA) and Salicylic acid (SA). It was observed that ABA, SA and tryptamine stimulated reserpine production in the whole plant cultures of R. serpentina while DMSO had a negative effect. Treatment with tryptamine (1.0 mg L−1) alone resulted in enhanced reserpine production (40.26 mg g−1 DW) while highest reserpine production (57.64 mg g−1) was obtained after 36 days of elicitation in cultures treated with 0.1 mmol SA plus 1.0 mg L−1 tryptamine. In the presence of ABA, SA or tryptamine alone or when a combination of SA plus tryptamine was used, the biomass production was reduced. Increase in reserpine production may be ascribed to synergistic effects of SA and tryptamine.
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