Stimulation of prostaglandin synthesis by ascorbic acid via hydrogen peroxide formation

Abstract

Ascorbic acid causes an increase in prostaglandin (PG) synthesis in human lung fibroblasts in culture. This is accompanied by an increase in fatty acid release from cellular lipid stores. The effects of ascorbic acid on prostaglandin synthesis and fatty acid release are erased if the cultures are treated simultaneously with catalase, but not if treated with superoxide dismutase. The action of catalase points to a role of hydroperoxides in the synthesis of prostaglandins. The addition of hydrogen peroxide itself increases prostaglandin synthesis by these cells.